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Efflux pump Rv1258c activates novel functions of the oxidative stress and via the VII secretion system ESX-3-mediated iron metabolic pathway in Mycobacterium tuberculosis 期刊论文

编号：

934BD9590BF2612575AD70C61E10669D
作者：

Sun, Hong(孙宏)^[1,2]Sheng, Gang^[1,2];Xu, Yuhui(徐玉辉)^[3]Chu, Hongqian(褚洪迁)^[1,2]Cao, Tingming(曹廷明)^[1,2]Dai, Guangming(戴广明)^[1,2]Tian, Na(田娜)^[1,2]Duan, Huijuan(段惠娟)^[1,2]Sun, Zhaogang(孙照刚)^[1,2]
语种：

英文
期刊：

MICROBES AND INFECTION ISSN：1286-4579 2024 年 26 卷 1-2 期 ; JAN-FEB
疾病分类：

肺结核PTB
关键词：

Mycobacterium tuberculosis Rv1258c ESX-3 Iron metabolism Oxidative stress
摘要：

Oxidative stress and iron metabolism are essential for Mycobacterium tuberculosis (M.tb) survival in host cells. The efflux pump Rv1258c belongs to the major facilitator superfamily (MFS) and can actively pump drugs to promote certain drug resistance in M.tb. In this study, we compared H37RvDRv1258c with wildtype (WT) M.tb H37Rv. The qRT-PCR results suggested that Rv1258c is potentially involved in the iron metabolic pathway by regulating the expression of ESX-3, which is required for iron uptake. Protein -Protein Affinity Predictor (PPA-Pred2) and the artificial intelligence program AlphaFold 2 were used for prediction and showed that Rv1258c has direct interactions with PPE4 and EccD3 but weak interactions with EccB3. This was further determined via protein-protein interaction analysis of the yeast two-hybrid expression system. By comparing mutant H37RvDRv1258c strains with WT strains, we discovered that the absence of Rv1258c led to elevated intracellular H+ potential and NAD+/NADH ratios in M.tb, thereby resulting in oxidative stress. We hypothesize that the efflux pump Rv1258c not only has the function of regulating drug resistance in M.tb but also has a novel function in activating oxidative stress and regulating ESX-3-associated iron metabolism in M.tb. (c) 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
推荐引用方式
GB/T 7714：

Sun Hong,Sheng Gang,Xu Yuhui, et al. Efflux pump Rv1258c activates novel functions of the oxidative stress and via the VII secretion system ESX-3-mediated iron metabolic pathway in Mycobacterium tuberculosis [J].MICROBES AND INFECTION,2024,26(1-2).
APA：

Sun Hong,Sheng Gang,Xu Yuhui,Chu Hongqian,&Sun Zhaogang.(2024).Efflux pump Rv1258c activates novel functions of the oxidative stress and via the VII secretion system ESX-3-mediated iron metabolic pathway in Mycobacterium tuberculosis .MICROBES AND INFECTION,26(1-2).
MLA：

Sun Hong, et al. "Efflux pump Rv1258c activates novel functions of the oxidative stress and via the VII secretion system ESX-3-mediated iron metabolic pathway in Mycobacterium tuberculosis" .MICROBES AND INFECTION 26,1-2(2024).
数据来源自科睿唯安Web of Science核心合集
入库时间：

2024/4/12 10:50:16
更新时间：

2024/4/12 10:50:16

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