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Molecular surveillance of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Mozambique, 2023 期刊论文

编号：

67A6FD8C9148874B10141AE01CF1ED8F
作者：

da Silva, Clemente^[1];Tembisse, Dario^[1];Cistero, Pau^[2];RoviraVallbona, Eduard^[2];Canana, Neide^[4];da Costa, Paulino^[1,4];Matambisso, Gloria^[1];ArandaDiaz, Andres^[3];Mbeve, Henriques^[1];Ndimande, Nelo^[1];Timana, Alcido^[1];Hunguana, Aura^[1];Boene, Simone^[1];Pujol, Arnau^[2];Rafael, Bernardete^[5];Greenhouse, Bryan^[3]Enosse, Sonia^[4];Saute, Francisco^[1];Candrinho, Baltazar^[5];Mayor, Alfredo^[1,2,6,7,8]
语种：

英文
期刊：

MALARIA JOURNAL ISSN：1475-2875 2024 年 23 卷 1 期 ; DEC 26
疾病分类：

疟疾
关键词：

Molecular surveillance Diagnostics RDT Malaria <italic>pfhrp2/3</italic> gene deletions Mozambique
摘要：

BackgroundRapid diagnostic tests (RDTs) based on the detection of Plasmodium falciparum histidine rich protein 2 (PfHRP2) are widely used for the diagnostic of P. falciparum in Africa. However, deletions of the pfhrp2 and pfhrp3 genes can lead to false negative test results and compromise appropriate case management. Due to the high burden of malaria in Mozambique, it is crucial to monitor the potential emergence of parasites with pfhrp2/3 gene deletions in the country.MethodsThe presence of pfhrp2/3 deletions was assessed during the 2023 high transmission season in 34 health facilities from 9 districts across 6 provinces in Mozambique. Children between 2 and 10 years of age attending the health facility with fever were tested by both the routine HRP2-RDT and a P. falciparum lactate dehydrogenase (PfLDH)-RDT, and dried blood spots (DBS) were collected from those testing positive by one or both RDTs. DBS from children with a negative HRP2-RDT but positive PfLDH-RDT were tested for the presence of pfhrp2/3 deletions by multiplex real time quantitative polymerase chain reaction (qPCR).Results3208 children attended the health facilities during the study. 81.6% (2612/3208) participants were positive for at least one malaria RDT and, among them, 0.8% (210/2612) had discrepant RDT results (22 HRP2 - but LDH + and 188 HRP2 + but LDH -). The overall prevalence of suspected false-negative HRP2-RDT results in Mozambique was 0.91% (95% CI 0.58-1.39; 22/2424). pfhrp2/3 gene deletions were confirmed in 4 cases (1 in Nampula and 3 in Inhambane). Therefore, the prevalence of P. falciparum confirmed cases with pfhrp2/3 gene deletions in the six provinces sampled was 0.16% (95% CI 0.15-2.57; 4/2424), being 0.27% (95% CI 0.01-1.75; 1/367) in Nampula and 0.59% (95% CI 0.15-1.88; 3/503) in Inhambane.Conclusionpfhrp2/3 gene deletions were detected in 2 out of 6 provinces surveyed in Mozambique, but at a prevalence far below the 5% threshold recommended for a change in HRP2-based-RDT.
推荐引用方式
GB/T 7714：

da Silva Clemente,Tembisse Dario,Cistero Pau, et al. Molecular surveillance of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Mozambique, 2023 [J].MALARIA JOURNAL,2024,23(1).
APA：

da Silva Clemente,Tembisse Dario,Cistero Pau,Rovira-Vallbona Eduard,&Mayor Alfredo.(2024).Molecular surveillance of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Mozambique, 2023 .MALARIA JOURNAL,23(1).
MLA：

da Silva Clemente, et al. "Molecular surveillance of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Mozambique, 2023" .MALARIA JOURNAL 23,1(2024).
数据来源自科睿唯安Web of Science核心合集
入库时间：

2025/1/3 18:00:29
更新时间：

2025/1/3 18:00:29

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