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Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor 期刊论文

编号：

4B95884C968CC701E8A84A5D393FF664
作者：

Yousefi, Meisam^[1] Lee, Wai Suet^[1] Chan, Wharton O. Y.^[1] He, Wei^[1] Mah, Marcus G.^[1] Yong, Cythia Lingli^[1] Deerain, Joshua M.^[2] Wang, Lijin^[3] Arcinas, Camille^[1] Yan, Biaoguo^[1] Tan, Dewei^[1] Sia, Wan Rong^[1] Gamage, Akshamal M.^[1] Yang, Jinxuan^[4] Hsu, Alan ChenYu^[1,5,6] Li, Shang^[7] Linster, Martin^[1] Yang, Xinglou^[4] Ghosh, Sujoy^[3,8] Anderson, Danielle E.^[2,9] Smith, Gavin J. D.^[1,12] Tan, Chee Wah^[1,10,12,14] Wang, LinFa^[1,9,12,14] Ooi, Yaw Shin^[1,11,12,13]
语种：

英文
期刊：

EMERGING MICROBES & INFECTIONS ISSN：2222-1751 2023 年 12 卷 2 期 ; DEC 8
疾病分类：

新型冠状病毒肺炎
关键词：

IGROV-1 COVID-19 betacoronavirus SARS-CoV-2 HCoV-OC43 Genome-scale CRISPR screening AHR DiMNF
摘要：

The emergence of novel betacoronaviruses has posed significant financial and human health burdens, necessitating the development of appropriate tools to combat future outbreaks. In this study, we have characterized a human cell line, IGROV-1, as a robust tool to detect, propagate, and titrate betacoronaviruses SARS-CoV-2 and HCoV-OC43. IGROV-1 cells can be used for serological assays, antiviral drug testing, and isolating SARS-CoV-2 variants from patient samples. Using time-course transcriptomics, we confirmed that IGROV-1 cells exhibit a robust innate immune response upon SARS-CoV-2 infection, recapitulating the response previously observed in primary human nasal epithelial cells. We performed genome-wide CRISPR knockout genetic screens in IGROV-1 cells and identified Aryl hydrocarbon receptor (AHR) as a critical host dependency factor for both SARS-CoV-2 and HCoV-OC43. Using DiMNF, a small molecule inhibitor of AHR, we observed that the drug selectively inhibits HCoV-OC43 infection but not SARS-CoV-2. Transcriptomic analysis in primary normal human bronchial epithelial cells revealed that DiMNF blocks HCoV-OC43 infection via basal activation of innate immune responses. Our findings highlight the potential of IGROV-1 cells as a valuable diagnostic and research tool to combat betacoronavirus diseases.
推荐引用方式
GB/T 7714：

Yousefi Meisam,Lee Wai Suet,Chan Wharton O. Y., et al. Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor [J].EMERGING MICROBES & INFECTIONS,2023,12(2).
APA：

Yousefi Meisam,Lee Wai Suet,Chan Wharton O. Y.,He Wei,&Ooi Yaw Shin.(2023).Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor .EMERGING MICROBES & INFECTIONS,12(2).
MLA：

Yousefi Meisam, et al. "Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor" .EMERGING MICROBES & INFECTIONS 12,2(2023).
数据来源自科睿唯安Web of Science核心合集
入库时间：

2023/10/16 10:19:41
更新时间：

2023/10/16 10:19:41

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