The study aimed to assess the diagnostic and prognostic value of 3 specific microRNAs (miRNAs) in early-onset neonatal sepsis (NS). We examined miR-1, miR-124, and miR-34a in 70 NS patients upon admission and compared them to 70 healthy controls by RT-PCR. The main finding of the study was the difference in miRNA expression levels between NS patients and controls. Higher expression levels of miR-1 and miR-124 were significantly associated with NS, while miR34a expression was reduced. Among the studied miRNAs, miR-34a exhibited the highest specificity (97%) as a confirmatory test for NS. In the multivariate model, miR-1 and miR-124 were found to be significant predictors of disease progression or mortality. Overall, the study suggests that miR-1, miR-124, and miR-34a could serve as potential biomarkers for diagnosing and predict-ing outcomes in early-onset NS. & COPY; 2023 Elsevier Inc. All rights reserved.
[1]Ain Shams Univ, Dept Clin Pathol Hematol, Fac Med, Cairo, Egypt.
[2]Ain Shams Univ, Ain Shams Med Res Inst MASRI, Fac Med, Cairo, Egypt.
[3]Egypt Ctr Res & Regenerat Med ECRRM, Dept Genet & Mol Biol, Cairo, Egypt.
[4]Armed Forces Coll Med AFCM, Dept Med Res, Cairo, Egypt.
[5]Zagazig Univ, Fac Med, Dept Med Biochem, Zagazig, Egypt.
[6]Princess Nourah Bint Abdulrahman Univ PNU, Fac Preparatory Year Hlth Sci, Dept Sci, Riyadh, Saudi Arabia.
[7]Ain Shams Univ, Fac Med, Dept Pediat, Cairo, Egypt.
[8]Mil Med Acad, Dept Med Microbiol & Immunol, Cairo, Egypt.
[9]Ain Shams Univ, Fac Sci, Dept Biochem, Cairo, Egypt.
[10]Ain Shams Univ, Fac Med, Dept Med Biochem & Mol Biol, Cairo, Egypt.
[11]October Univ Modern Sci & Arts MSA, Fac Biotechnol, Giza, Egypt.
[12]Sinai Univ, Fac Dent, Biochem Dept, Kanatra, Egypt.
(8.0+极速模式)