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Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements 期刊论文

编号：

2740097039F735BF4C199CD3D23AB511
作者：

Berdichevski, Mayana Kieling Hernandez^[1,2] Guerra, Rafaela R.^[2,3] Pereira, Dariane C.^[2] Wilhelm, Camila M.^[2] Barth, Patricia O.^[2] Silveira, Melise C.^[4] Volpato, Fabiana C. Z.^[2] RochadeSouza, Claudio^[4] Carrassai, Richard M.^[2] CarvalhoAssef, Ana Paula^[4] Martins, Andreza F.^[1,2,3] Barth, Afonso Luis^[1,2]
语种：

英文
期刊：

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE ISSN：2213-7165 2025 年 42 卷 (214 - 221) ; MAY
关键词：

Co-integrated plasmids BlaKPC-2 BlaNDM-1 K. pneumoniae co-harbouring Bioinformatics Genomic surveillance
摘要：

Objective: Infections due to carbapenemase-producing Enterobacterales harbouring more than one carbapenemase-encoding gene spread mainly by plasmid and transposon mobilisation. The objective of this study was to analyse the mobile genetic elements carrying blaKPC and blaNDM of Klebsiella pneumoniae carbapenemase co-producers (KpKN). Methods: K. pneumoniae isolates with reduced susceptibility to carbapenems were obtained between 2016 and 2023. To evaluate the genetic environment of KpKN, 22 isolates were selected for antimicrobial susceptibility testing and whole-genome sequencing. Results: The blaKPC-2 gene was carried mainly by IncN/IncFIB, a novel co-integrated plasmid in the Tn4401b transposon. blaNDM-1 was disseminated in the only two KpKN isolates recovered prior to 2020 by the IncHI1B/IncFIB plasmid type within the Tn3000 transposon. Significantly, isolates obtained since 2020 showed the blaNDM-1 gene carried by IncA/C in an IS26-flanked pseudo-composite transposon containing ISCR1, which also had genes that conferred resistance to sulfonamides, aminoglycosides, macrolides, quinolones, amphenicols, tetracyclines, rifampicin, sulfonamide, and trimethoprim. The isolates belonged mainly to ST11and ST16. Conclusions: Plasmid and transposon changes during different periods could be related to higher dissemination of blaNDM-1, and the large number of resistance genes present in the IS26-flanked transposon may have increased co-selection of this plasmid through the wide use of antimicrobials during the pandemic. (c) 2025 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
推荐引用方式
GB/T 7714：

Berdichevski Mayana Kieling Hernandez,Guerra Rafaela R.,Pereira Dariane C., et al. Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements [J].JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE,2025,42:214-221.
APA：

Berdichevski Mayana Kieling Hernandez,Guerra Rafaela R.,Pereira Dariane C.,Wilhelm Camila M.,&Barth Afonso Luis.(2025).Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements .JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE,42:214-221.
MLA：

Berdichevski Mayana Kieling Hernandez, et al. "Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements" .JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE 42(2025):214-221.
数据来源自科睿唯安Web of Science核心合集
入库时间：

2025/5/14 12:00:06
更新时间：

2025/5/14 12:00:06

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